不同催产剂对俄罗斯鲟人工繁殖效果的影响

摘要

Three kinds of hormones were evaluated based on the results of artificial propagation in cultured Russian sturgeon .Injection of carp pituitary ,L HRH-A2 and L HRH-A2 +DOM induced cultured sturgeon brood fish mature and spawn .Among them ,the combination of LHRH-A2 +DOM was the most effective hormones based on results including latency time (22-38 h) ,success rate(80% ) ,fertilization rate (83 .2 ± 3 .46)% and hatching rate(83 .2 ± 3 .46)% .There was no significant difference in induction of sperm and quality among difference experimental groups .Duration of sperm quick movement was 58-115 s ,the life span of sperm was 130-235 s ,and the sperm density was 0 .1 × 109 -2 .21 × 109/mL .%分别给性腺发育成熟的俄罗斯鲟亲鱼注射鲤鱼脑垂体、促黄体素释放激素类似物以及促黄体素释放激素类似物＋马来酸地欧酮进行人工繁殖。试验结果表明，3种激素均能在水温15～16℃下诱导人工养殖雌鱼卵成熟与排出，其中注射促黄体素释放激素类似物＋马来酸地欧酮组的效果最好：效应时间22～38 h ，产卵率80％，受精率（83．2±3．46）％，孵化率（82．1±3．85）％。不同催产剂对雄鱼精液的排出和质量影响无明显差异。试验组不同雄鱼个体的精子快速移动时间为58～115 s ，寿命为130～235 s ，精子密度为0．1×109～2．21×109个/m L。