＜正＞ Objective: To evaluate the killing effect of photodynamic therapy （PDT） using new photosensitizer benzoporphyrin derivative complexed with human low density lipopro-tein on retinoblastoma （RB） cell line in vitro.Methods: The experiment of photodynamic killing effect on RB cell line in vitro was performed by using benzoporphyrin derivative （BPD or Verteporfin） and monochromatic light at the wavelength around 690nm. Seven BPD concentrations （2 500ng/ml, 1250ng/ml, 625ng/ml, 312. 5ng/ml, 156.25ng/ml, 78.125ng/ml, 39.062 5ng/ml） and three energy densities （1. 2J/cm2, 2. 4J/cm2 and 3. 6J/cm2） were applied. The damage of the tumor cells was evaluated by MTT assay 24 hours after PDT. The changes of the ultrastructure of RB cells were observed under eleceronic microscope 4 hours after PDT.Results: There was a significant dose-response relationship between tumor cell damage and BPD concentration in the medium under light irradiation at the energy density of 1.2J/cm . At each BPD concentration, the inhibition rat
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