高效液相色谱法测定柴芍胃炎颗粒中芍药苷的含量

摘要

目的:采用高效液相色谱法测定柴芍胃炎颗粒中芍药苷含量.方法:色谱柱为ZORBAX SB-C18柱(250 mm×4.6 mm,5 μm),流动相为乙腈-0.1%磷酸溶液(V∶V=22∶78),检测波长为230 nm,流速为1.0 ml/min,柱温为30 ℃.结果:芍药苷的质量浓度在49.68~186.3 μg/ml范围内与峰面积呈良好线性关系(r=0.999 7,n=5),芍药苷质量浓度的平均加样回收率为96.05%,RSD为0.56%(n=6).结论:本方法简便、灵敏、检测结果准确,可用于柴芍胃炎颗粒的质量控制.%OBJECTIVE:To establish a method for content determination of paeoniflorin in Chaishao gastritis granules by HPLC. METHODS:The column was ZORBAX SB-C18 column(250 mm×4.6 mm, 5 μm), the mobile phase consist of acetonitrile-0.1% phosphoric acid solution(V∶V=22∶78), with detective wave length of 230 nm, flow rate of 1.0 ml/min and column temperature of 30 ℃. RESULTS:The calibration curve of paeoniflorin was linear over the range of 49.68 μg/ml-186.3 μg/ml (r=0.999 7,n=5). The average recovery rate was 95.05%,RSD was 0.56% (n=6). CONCLUSIONS:This method is simple,sensitive, accurate and reliable, and can be used for the quality control of Chaishao gastritis granules.