荧光法测定悬浮培养藻体叶绿素含量的研究

摘要

Laboratory always obtain the content of algae volume by measuring the chlorophyll a fluorescence intensity. According to the settlement of algae cells, fluorescence value are greatly influenced by static duration, when the static duration is 1min, attenuation rate of fluorescence was ΔF/F0=-9�96% and the determination of algae density is not exact. In the article, a method of algae culture preparation was established by adding agar powder(0�08~0�10g/100mL) to the culture. The suspensibility and stability of the culture was enhanced dramatically by this method, after standing 1 hour, attenuation rate of fluorescence was ΔF/F0=-4�82%≤± 5%�The method enhance the accuracy of chlorophyll contents measurement by chlorophyll spectrofluorimetry and reduces the effect of culture sedimentation to the measurement.%实验室常采用荧光检测技术实现对藻的定量检测。藻细胞沉降，导致其荧光值受静置时间影响较大，静置1min时，荧光值的衰减率达ΔF／F0＝－9�96％，无法获得准确的藻浓度。因此，本文提出一种藻体样品制备方法，培养过程中添加琼脂粉（0�08～0�10g／100mL）作为藻液悬浮稳定剂。用该方法培养的藻液其悬浮性、稳定性均有明显提高，且静置1h后，ΔF／F0＝－4�82％≤±5％。减少了藻体沉降对测定结果的影响，确保了荧光光度法测定叶绿素含量的准确性。