Genus Ostreopsis Schmidt is widely distributed in the benthic marine ecosystem. Some of them can produce palytoxin and its analoges (PLTXs), which are highly toxic, causing human poisoning through inhalation of contaminated aerosols or consumption of contaminated seafood. There is still no report about toxin produced by Ostreopsis so far in China. In this study, two strains of Ostreopsis sp., WZD110 and WZD111, were isolated from Weizhou island, China. The toxicity test and toxin analysis were conducted. The results from the mouse bioassay showed the symptoms of severe posterior lumbar depression, decreased locomotion, convulsions and acute death after being injected intraperitoneally with crude algal extracts. The hemolysis neutralization analysis revealed that the hemolysis effects of the crude algal extracts were inhibited effectively by Ouabain, a PLTXs antagonist. The toxins have also been analyzed qualitatively and quantitatively by LC-MS/MS and LC-HRMS. The results indicated that the PLTXs content of strain WZD110 and WZD111 were 0.348 pg·cell-1 and 0.081 pg·cell-1, respectively, both containing higher concentrations of OVTX-c and OVTX-d/e, and maybe small amount of OVTX-g and OVTX-f. This is the first report on the toxin study on Ostreopsis from Chinese Sea, providing some scientific references on understanding of the harm done by Ostreopsis bloom.%蛎甲藻属(Ostreopsis Schmidt)部分种类可以产生剧毒的海葵毒素(Palytoxins,PLTXs),对海洋生态环境和人类健康构成严重威胁,而国内迄今尚无关于蛎甲藻毒性的研究报道,因此对采自广西涠洲岛同一种蛎甲藻的两个株系(WZD110、WZD111)进行了产毒特性研究.在小鼠生物毒性实验中,腹腔注射两株藻的毒素粗提取物后,小鼠均出现后腰凹陷、运动减少和抽搐等症状,并于短时间内死亡,呈现明显的PLTXs中毒特征.兔血细胞溶血活性实验结果表明,毒素粗提取物具有延迟溶血的效果,且在加入PLTXs特异抑制剂乌本苷后,延迟溶血活性被抑制.液相色谱-质谱联用毒素定性定量分析结果显示,WZD110株单位细胞PLTXs质量为0.348 pg·cell-1,WZD111株单位细胞PLTXs质量为0.081 pg·cell-1,两株系均含有较高浓度的OVTX-c和OVTX-d/e,以及少量的OVTX-g和OVTX-f.文章关于蛎甲藻胞内毒素的报道在中国海域的此类研究尚属首次,可为正确认识和评价蛎甲藻赤潮产生的危害提供参考依据.
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