二十五味珊瑚丸中马兜铃酸A含量研究

摘要

Objective: To develop a high performace liquid chromatography (HPLC) method for the determination of aristolochic acid A in Ershiwuweishanhu Pill and to use the method in the qualitative and quantitative determination of aristolochic acid A in Ershiwuweishanhu Pill purchased from market. Methods: The column used was kromasil C18 (4.6×200 mm), and methanol -0.05% glacial acetic acid (62:38) was used as the mobile phase. The column temperature was set at 30℃, the flow rate was 1 mL/min and the detection wavelength was set at 250 nm. Results: The calibration curve of aristolochic acid A was linear in the range of 0.052~0.0936 mg/mL (r=0.9999). The average recovery was 99.67%. RSD wass 1.33%. Conclusion: The established method is convenient, fast, accurate, sensitive and repeatable.%　　目的：建立二十五味珊瑚丸中马兜铃酸A含量的HPLC测定方法，用于市场上二十五味珊瑚丸中马兜铃酸A的定性和定量检测。方法：采用kromasil C18(4.6×200mm)，甲醇-0.05%冰醋酸(62：38)作为流动相，柱温30℃，流速1mL/min,250nm波长下检测。结果：马兜铃酸A在0.052mg/mL-0.0936mg/mL范围内线性关系良好，r=0.9999。平均回收率为99.67%，RSD为1.33%。结论：以高效液相色谱法检测二十五味珊瑚丸中马兜铃酸A的方法简便、快速、准确、灵敏度高，重复性良好。