Betaine was accumulated as a nontoxic andprotective osmolyte in water-dificit and salt-stressed plants.Be-taine aldehyde dehydrogenase for glycine betaine synthesis is an important enzyme.The BADH gene of Maizewas cloned by RT-PCR and RACE.The gene is 1 762 bp in length,including an open reading frame of 1 515 bp.Its nucleotide sequence shares 95% with the the partial cDNA of BADH1 from Sorghum bicolor.Its deducedamino acid sequence contains the conserved domain sequence 'VTLELGGKSP' of ALDH,and a tripeptide SKLat its C-terminal,a signal targetting to the microbodies.The phylogenetic tree of 20 BADHs was constructed,which corresponds to the classical botanical division of plant,the BADH of maize is closest to the one of Oryza Sativa.GenBank accession No.AY587278.
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