Anti-Helicobacter pylori effect of CaG-NANA, a new sialic acid derivative

摘要

AIM To investigate the bactericidal effects of calcium chelated N-acetylneuraminic acid-glycomacropeptide(CaG-NANA) against Helicobacter pylori(H.pylori).METHODS For manufacture of Ca G-NANA,calcium(Ca) was combined with glycomacropeptide(GMP) by chelating,and N-acetylneuraminic acid(NANA) was produced with Ca-GMP substrate by an enzymatic method.The final concentration of each component was 5% Ca,7% NANA,85% GMP,and 3% water.For in vitro study,various concentrations of CaG-NANA were investigated under the minimal inhibitory concentration(MIC).For in vivo study,Ca G-NANA was administered orally for 3 wk after H.pylori infection.The levels of inflamma-tory cytokines in blood were analyzed by enzyme-linked immunosorbent assay and eradication of H.pylori was assessed by histological observation.RESULTS The time-kill curves showed a persistent decrease in cellnumbers,which depended on the dose of CaG-NANA,and MIC of Ca G-NANA against H.pylori was 0.5% in vitro.Histopathologic observation revealed no obvious inflammation or pathologic changes in the gastric mucosa in the CaG-NANA treatment group in vivo.The colonization of H.pylori was reduced after CaG-NANA treatment.The levels of interleukin(IL)-6,IL-1β,tumor necrosis factor-α,and IL-10 were also decreased by CaG-NANA.CONCLUSION Ca G-NANA demonstrates effective anti-bactericidal activity against H.pylori both in vitro and in vivo.