Objective:Ultrarapid freezing method (vitrification) is an alterna tive method for cryopreserving ovarian tissue. The feasibility of int act murine ovaries cryopreservation was studied by vitrification.Methods: Intact mouse ovari es were cryopreserved using vitrification method and slow freezing method. The m orphological alterations after frozen-thawed procedure and the apoptosis rates o f primordial follicles were evaluated by histological examniation and TUNEL tech nique, and transmission electron microscopy (TEM) was used to observe the ultras tructural changes.Results:The percentages of normal primordial fol licles in fro zen-thawed groups were significantly lower than that in the control group (P0. 05) in these two groups. In the vitrification group, slow freezing group and the fresh control group, the percentages of the TUNEL-positive follicles were 16.3 7 %, 13.29% and 21.36%, respectively. The apoptosis rates in the two cryopreservat ion groups showed no significant difference (P>0.05). After the freezing-thawing procedure, subcellular structure was well preserved in both freezing groups wit h smililar ultrastructural changes.Conclusion:Vitrification is a convenient and efficient approach for small ovarian tissue cryopreservation.
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