This study compared the accumulation of Zn2+and Cu2+in the ovaries and ova of loaches under different concentrations of Zn2+(1.00, 2.50 and 5.00 mg/L respectively) and Cu2+(0.10, 0.25 and 0.50 mg/L respectively). The results showed that both Zn2+and Cu2+accumulated in the ovaries, and that the relationship between accumulation and time was linear over 20 days of exposure. The accumulation of the metals in ovaries was closely related to the concentration of exposure in the solutions(P<0.05), and was obviously affected by the time and doses. However, the Cu2+concentration was significantly higher than Zn2+(P<0.05). The development level of ova in the ovaries also correlated with the concentration and exposure period in the Zn2+and Cu2+solutions.
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