Objectives Recent evidence have implicated specific gene polymorphisms of arachidonate 5-lipoxygenase-activating protein(ALOX5AP),and 2 at-risk haplotypes (HapA,HapB ) in myocardial infarction(MI) and stroke, whereas other studies have been conflicting and to date,no data concerning coronary artery disease(CAD) are available in the Han population of North China.The aim of the present study was to investigate the possible associations between the variants of ALOX5AP gene and susceptibility to CAD in the Han population of North China.Methods We sequenced the promoter,all of the exons,splite site region and 3’untranslated region of ALOX5AP gene,and 7 single nucleotide polymorphisms(SNPs) were found.Three(-1340T/G,+ 8733T/C,+20616G/C) of these polymorphisms were evaluated in 656 patients with angiographically proven CAD and 678 controls with normal coronary angiograms using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) assay.Allelic,genotypic and haplo-typic association testing were performed using Shesis and Phase version 2.1 software package.Multiple logistic regression was used to control for the presence of vascular risk factors.Results While none of the single nucleotide polymorphism (SNP) was found to be associated with CAD risk individually,there was an association with the 3 SNP haplo-types. We identified a protective haplotype -1340T,+8733C and +20616G(Bonferroni-corrected P=0.000002984,OR = 0.623,95%CI=0.519-0.748) and a risk haplotype -1340G, +8733C and +20616G(Bonferroni-corrected P =0.000018, OR=1.728,95%CI=1.375-2.171).The frequencies of all alleles were in Hardy |= Weinberg equilibrium.Conclusions Our data suggest that unique haplotype combinations in the ALOX5AP gene may play a role in the etiology of CAD.
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