Microelectrode Array-evaluation of Neurotoxic Effects of Magnesium as an Implantable Biomaterial

摘要

Magnesium(Mg)-based biomaterials have shown great potential in clinical applications. However,the cytotoxic effects of excessive Mg^(2+) and the corrosion products from Mg-based biomaterials,particularly their effects on neurons,have been little studied. Although viability tests are most commonly used,a functional evaluation is critically needed. Here,both methyl thiazolyl tetrazolium(MTT) and lactate dehydrogenase(LDH) assays were used to test the effect of Mg^(2+) and Mg-extract solution on neuronal viability.Microelectrode arrays(MEAs),which provide long-term,real-time recording of extracellular electrophysiological signals of in vitro neuronal networks,were used to test for toxic effects. The minimum effective concentrations(EC_(min)) of Mg^(2+) from the MTT and LDH assays were 3 mmol/L and 100 mmol/L,respectively,while the EC_(min) obtained from the MEA assay was 0.1 mmol/L. MEA data revealed significant loss of neuronal network activity when the culture was exposed to 25% Mg-extract solution,a concentration that did not affect neuronal viability. For evaluating the biocompatibility of Mg-based biomaterials with neurons,MEA electrophysiological testing is a more precise method than basic cell-viability testing.