听源性遗传癫痫易感大鼠大脑皮层胆囊收缩素mRNA的原位杂交检测

摘要

Using in situ hybridization method,we examined CCK mRNA positive signal in temporal cerebral cortex of audiogenic seizure-prone rats（P77PMC）before and after a single or multiple seizures.The results were as follows：(1)CCK mRNA levels were elevated significantly in temporal cortex after a single or multiple seizures；(2)CCK mRNA level in multiple consecutive seizure rats was lower than that in a single seizure rats. The increased CCK mRNA positive signal indicates that CCK mRNA in cerebral cortex may play a role in seizure-associated processes. The decreased CCK mRNA levels after multiple consecutive seizures may suggest differential regulation of CCK transcription by single and multiple seizures.%本实验用原位杂交法，对听源性遗传癫痫易感大鼠（P77PMC）癫痫发作前、单次癫痫发作和多次发作时大脑颞皮层CCK mRNA阳性信号进行了检测。结果显示：（1）单次和多次癫痫发作后颞皮层CCK mRNA 阳性神经元数量明显增加 (P<0.01)；（2）多次癫痫发作者上述脑区CCK mRNA 阳性神经元数量较单次癫痫发作有明显的下降(P<0.01)。大脑颞皮层CCK mRNA增高表明，CCK mRNA在癫痫发作过程中起了某种作用；多次癫痫发作大鼠CCK mRNA表达降低提示，单次和多次癫痫发作时大脑皮层对CCK mRNA转录的调控可能存在不同的机制。