Breeding and Simple-rapid Regeneration Protocol for Jisheng 1 with Glandless Trait and High-frequency Somatic Embryo Production Ability

摘要

@@ Most of model cotton varieties used in tissue culture have glands on both the reproductive and vegetative parts of the plant.These glands contain compounds that are toxic to human and non-ruminant animals.The presence of these compounds limits their usage as food and feed.To obtain a glandless cotton variety with high-frequency somatic embryo production ability,27 glandless varieties were screened in improved MSB media containing 0.1 mg · L-1 IAA,0.1 mg · L-1 KT,and 0.1 mg · L-1 2,4-D.Hengwu 8930,whose regeneration rate was only 2.5%,and the cycle of regeneration was about 12 months had the highest frequency.