将(亚洲棉×比克氏棉)F_1加倍,再与陆地棉、海岛棉、(陆地棉×夏威夷棉)、(陆地棉×黄褐棉)杂交,获得三种杂种与四种杂种.本研究用RAPD技术对种间杂种及其亲本进行了分析,结果表明:(1)15个引物对参试材料总共扩增出127条DNA带,其中有102条多态性带,占80.3 %;(2)在杂种中均检测到了亲本带与杂种的特异带;(3)利用SAS软件中的类平均法对试验材料进行聚类分析,结果表明,所有的参试材料聚为三大类.研究结果充分验证了杂种的真实性.%The interspecific hybrids were derived from ［(Gossypium arboreum×Gossypium bickii) × Gossypium hirsutum］, (G. arboreum×G.bickii)×Gossypium barbadense］, ［(G. arboreum×G. bickii) × (G.hirsutum×Gossypium mustelinum)］, (G. arboreum×G.bickii) × (G. hirsutum×Gossypium tomentosum)］ after doubling F_1 of (G. arboreum×G.bickii). These hybrids and their parents were analyzed by RAPD. The results showed that: （1）There were 127 amplified DNA bands from 15 primers, 102 bands (80.3 %) were polymorphic; (2) Parents bands and specific bands of hybrids were detected in hybrids; (3) The cluster analysis of materials was studied by group-average method from SAS software. Cluster analysis revealed that these materials were divided into 3 groups. All the results verified the authenticity of hybrids.
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