[目的]旨在深入挖掘大丽轮枝菌致病相关基因.[方法]运用转录组测序技术比较分析了强致病力大丽轮枝菌Vd991在侵染陆地棉品种Coker 312早期阶段的基因表达情况.[结果]对Vd991侵染前后的转录组数据进行差异表达分析,共筛选到979个差异表达基因(Differentially expressed genes,DEGs),其中376个上调,603个下调.通过基因注释和功能分类发现,这些DEGs涉及细胞增殖与生长、产孢、碳水化合物结合、蛋白激酶活性调节、裂解酶活性、水解酶活性等功能.对上调DEGs进行基因本体(Gene ontology,GO)功能富集分析,发现共有277个GO词条达到显著富集水平(P＜0.05),涉及生长速率正向调控、核苷酸合成、解旋酶活性等功能;京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路分析表明,上调DEGs参与53个代谢通路,包括嘧啶代谢、嘌呤代谢等.[结论]在早期侵染过程中,大丽轮枝菌细胞增殖相关基因表现活跃.上述分析为进一步揭示大丽轮枝菌的致病机理提供了有价值的参考.%[Objective] The aim of this study is to mine pathogenesis related genes of Verticillium dahliae.[Method] We explored the transcriptome of highly toxic V.dahliae strain Vd991 at the early stage of infection in Coker 312,an upland cotton cultivar.[Result] Through differential expression analysis,a total of 979 differentially expressed genes (DEGs)were identified after infection,of which 376 were up-regulated and 603 were down-regulated.The result of gene annotation and classification showed that these DEGs are involved in broad biological processes,molecular function and cellular component,including reproductive process,growth,sporulation,carbohydrate binding,kinase regulator activity,lyase activity and hydrolase activity.Enrichment analysis of the up-regulated DEGs were performed,277 gene ontology terms were enriched,including positive regulation of growth rate,purine nucleotide biosynthetic process,pyrimidine nucleobase biosynthetic process,helicase activity.A total of 53 Kyoto Encyclopedia of Genes and Genomes pathways were identified in the up-regulated DEGs,including purine metabolism,pyrimidine metabolism.[Conclusion] These results indicate that genes related to cell proliferation actively expressed at the early stage of infection and provide valuable reference for exploring the pathogenic mechanism of V.dahliae.
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