Let {A,B} be a complete system of the closed orientable surface F of genus 2. A simple closed curve C on F is separating with respect to (A, B) if it is disjoint from A∪B and it cuts F into two once-punctured tori X, Y with A(?)X, B(?)Y. Letγbe a simple closed curve on F which is disjoint from A∪B and intersects C essentially in two points. In this paper, we show that up to isotopy, {hnγ(C):n∈Z} is the set containing all the simple closed curves on F which is separating with respect to (A,B), where hγis the Dehn twist alongγon F. This also shows how two simple closed curves on F which are separating with respect to (A,B) are related. The result can be applied to yield all Haken spheres of a Heegaard splitting V∪F W which are weakly equivalent to a given Heken sphere of the splitting.
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