Objective: To investigate the prevalence of Helicobacter pylori (H. pylori) infection in dyspepsia patients and its relation to virulence factor cagA gene. Methods: In total, 110 gastric biopsies from dyspeptic patients were comparatively studied using rapid urease test and multiplex polymerase chain reaction (PCR). Results: Multiplex PCR detected three genes of 16S rRNA, cagA, and ureC. H. pylori was detected in 14 gastric biopsies (13%). Significantly higher numbers of female were infected. Furthermore,cag A gene was found in all H. pylori-positive specimens. In addition, the result indicated that the multiplex PCR with annealing temperature at 57 oC was able to effectively amplify specific products. Conclusions:The results confirmed high prevalence of cagA gene in H. pylori among dyspeptic patients in Southern Thailand.
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