Objective To study the mechanism of pre-B-cell colony enhancing factor (PBEF) on increasing of pulmonary vascular endothelial permeability after cardiopulmonary bypass (CPB) operation and to provide theoretical basis for protection of lung injury during CPB. Methods Conventional animal models were established and grouped. The control group animals were established CPB after anesthesia without CPB transfer. Group A was transfected by lentivirus AD-PBEFshRNA and established CPB after anesthesia without CPB transfer. Group B was for 30-minute deep low temperature cycle after CPB. Group C was transfected by lentivirus AD-PBEFshRNA and then for 30-minute deep low temperature cycle. Every group above was 10 animals. Phosphorylated P38MAPK, ERK, MLC, VE-cadherin and FAK in each group was detected by Western blotting. Serum levels of TNF-αand IL-6 for two groups were detected by ELISA method. Results Expressions of mRNA in PBEF, P38MAPK, ERK, MLC, VE-cadherin and FAK in group C were evidently higher than those in group A and B (P<0.01). Serum levels of TNF-αand IL-6 in group C were obviously higher than those in group A and B, with statistical differences (P<0.01). Conclusions PBEF can increase pulmonary vascular endothelial permeability after CPB operation by pathways of P38MAPK and ERK. And TNF-αand IL-6 may involve in this process.%目的 研究体外循环 (CPB) 术后前B细胞克隆增强因子 (PBEF) 在肺血管内皮通透性增加中的机制, 为CPB期间肺损伤保护提供理论依据. 方法 常规动物模型建立并进行分组, 对照组动物麻醉后建立CPB, 不行CPB转流; A组动物进行慢病毒AD-PBEFshRNA转染, 麻醉后建立CPB, 不行CPB转流; B组动物建立CPB后进行30 min深低温停循环; C组动物慢病毒AD-PBEFshRNA转染后, 再进行30 min深低温停循环; 以上每组10只动物. RT-PCR法检测各组PBEF mRNA; West-ern blotting检测各组磷酸化P38MAPK、 磷酸化ERK、 磷酸化MLC、 磷酸化VE-cadherin以及磷酸化FAK表达; ELISA法检测各组动物血清肿瘤坏死因子 (TNF) -α和白细胞介素 (IL) -6水平. 结果 C组PBEF的mRNA表达、 P38MAPK、 ERK、 MLC、VE-cadherin和FAK蛋白表达均明显高于A组和B组, 差异均有统计学意义 (P<0.01); C组血清TNF-α和IL-6水平均高于A组和B组, 差异均有统计学意义 (P<0.01). 结论 PBEF通过P38MAPK、 ERK等途径增加CPB术后肺血管内皮通透性, TNF-α和IL-6也可能参与了这一过程的调控.
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