PRDX1在结直肠癌中的表达及其临床意义

摘要

目的 探讨过氧化氧化还原蛋白1(PRDX1)在结直肠癌组织及远处正常组织中的表达情况及其与患者临床病理资料间的关系.方法 采用免疫组织化学对32例结直肠癌组织及相应的远处正常组织中PRDX1的表达情况进行检测,随机抽取8例标本采用蛋白质印迹的方法进行验证;将PRDX1的表达情况与患者的性别、年龄、肿瘤分期、淋巴结转移等临床病理资料进行统计学分析.结果 免疫组织化学显示,PRDX1蛋白主要表达于细胞质,在结直肠癌远处正常组织中的表达(15.63%,5/32)与结直肠癌组织中(65.63%,21/32)比较,差异有统计学意义(P＜0.01).蛋白质印迹的验证结果与免疫组织化学结果一致.PRDX1蛋白的表达与患者的性别、年龄间无明显相关性(P>0.05),然而PRDX1蛋白的表达与Ⅲ期结直肠癌患者或者有淋巴结转移的患者之间有明显的相关性(P＜0.05).结论 PRDX1在结直肠癌组织中高表达,可能参与了肿瘤的进展,检测其表达情况在结直肠癌的诊治中可能具有一定的作用.%Objective To investigate the expression of peroxiredoxin 1(PRDX1) and its clinical significance in histological samples of colorectal neoplasm and the distant normal tissues. Methods In 32 cases of colorectal cancer, the expression of PRDX1 in histological samples of colorectal neoplasm and the distant normal tissues was detected by immunohistochemistry. A total of 8 tumor specimens and a corresponding nonmalignant sample of colorectal from distant areas of the same patient were selected for western bolt analysis,and the correlations with patient clinical and pathological data such as sex,age,stage and lymphnodes metastasis were analyzed. Results Immunohistochemistry indicated that PRDX1 protein was expressed mainly in the cytoplasm. In distant normal colorectal tissues(15. 63% ,5/32) showed weak expression of PRDX1 compared with it in carcinomas tissue(65. 63% , 21/32) ,the differences had statistical significance(P<0.01). In line with the immunohistochemistry, western blot analysis revealed PRDX1 protein was significantly higher in carcinomas than in control colorectal tissues. When comparing PRDX1 with clinicopatho-logical parameter,it did not have significant association with sex or age(P>0.05). However,PRDX1 showed significantly increased expression in colorectal cancer patients with stage III or lymphnodes metastasis-positive cases(P<0.05). Conclusion In colorectal tumors,the expression of PRDX1 is higher; it might be involved in tumor progression and played a role in diagnosis and treatment of colorectal cancer.