非甾体类抗炎药NAG-1在胃癌中表达及意义

摘要

Objective To study the expression of nonsteroidal anti inflammatory drug - activated gene-1 in human gastric cancer and its relationship with the clinical staging,differentiation degree,and lymphatic metastasis of gastric cancer.Methods The expression of NAG-1 was detected by semiquantitative reverse transcriptase polymerase chain Reaction ( RT-PCR) and immunohistochemistry(IHC) in the cancer tissues and corresponding adjacent normal tissues in 76 gastric cancer patients.Results Immuno-histochernistry showed the expression of NAG-1 protein in the cancer tissues was lower than that in the normal tissues (x2 = 47.186 ,P＜0.05) ; and the expression was relationship with differentiation degree( x2 = 7.506 ,P＜0.05) , but the expression was not associated with the depth of tumor invasion,lymph node metastasis, and clinical stage(all P＞0.05).The result of RT-PCR showed that NAG-1 mRNA expression in gastric cancer tissue significantly was lower than that in normal tissues(P＜0.05).NAG-1mRNA expression in well and moderately differentiated cancer cells was significantly higher than those of poorly and non-differerntiated cancer cells(P＜0.05) , the expression was not associated with the depth of tumor invasion, lymph node metastasis, and clinical stage(P＞0.05).Conclusion Decreased expression of NAG-1 in human gastric cancer,and it may play an important role in the tumorigenesis of gastric cancer.%目的 研究非甾体类抗炎药活化基因-1(NAG-1)在胃癌组织中的表达及其与临床病理特征的关系.方法 采用RT-PCR和免疫组化检测76例胃癌组织及其相应正常组织NAG-1的表达.结果 胃癌组织的NAG-1蛋白的表达程度显著低于正常组织(χ2=47.186,P＜0.05).NAG-1蛋白在胃癌组织中的表达与患者的细胞分化程度有关(χ2=7.506,P＜0.05).而与性别、淋巴结转移、临床分期间的差异无统计学意义(P＞0.05).RT-PCR结果显示NAG-1 mRNA在胃癌组织中的相对含量显著低于正常胃黏膜组织.NAG-1 mRNA在高、中分化胃癌中的相对含量显著高于低、未分化(P＜0.05).而与淋巴结转移、临床分期、浸润深度差异无统计学意义(P＞0.05).结论 NAG-1基因在胃癌组织中的表达下降,可能参与胃癌的发生、发展过程.