神经生长因子和表皮生长因子在衰老大鼠下颌下腺的表达

摘要

Objective To observe the expressions of NGF and FGF in submandibular glands of aging rats induced by D-galacrose.Methods 18 female SD rats were randomly divided into normal and model groups.D-galactose senescence model was adopted in this research by continuous intraperitoneal injection of D-galactose for 60 days.SP immunohistochemistry technology was used to observe the expressions of NGF and EGF in submandibular glands.Results NGF and EGF immunopositive products were brown or dark brown granules and mainly appeared in the apical cytoplasm of GCT and secreting duct cells.NGF and EGF expressing in submandibular gland cells in the normal group were significantly higher than those of model group(P＜0.01).Conclusion The expressions of NGF and EGF in.aging submandibular glands decrease obviously.It provides experimental data for the research on delaying tissue regression and prevention of aging submandibular gland disease.%目的 观察D-半乳糖所致衰老大鼠下颌下腺神经生长因子(NGF)、表皮生长因子(EGF)表达的改变.方法 选取18只SD雌性大鼠,随机分成正常组、模型组,模型组大鼠每天腹腔注射D-半乳糖,连续60 d,制造亚急性衰老大鼠模型.用SP免疫组化法检测下颌下腺NGF、EGF的阳性表达.结果 NGF、EGF阳性反应颗粒为浅棕色至深褐色,主要分布于颗粒曲管细胞及纹状管细胞顶部胞质中,腺泡细胞为阴性.正常组NGF、EGF表达明显高于模型组(P＜0.01).结论 D-半乳糖所致衰老大鼠下颌下腺NGF、EGF表达较正常大鼠明显减少,说明NGF、EGF表达伴随衰老明显降低,为延缓组织退行性改变、预防衰老性涎腺疾病研究提供了一定的实验资料.