目的 研制一种通过微板干试剂葡萄糖氧化酶法快速检测血糖的新方法,并小批量在普查糖尿病中应用.方法 将改良葡萄糖试剂冻干于96孔板中并封存.检测时微板中每孔由排枪加入200∶1比例去离子水和待检标本混匀的预处理液,复溶冻干试剂并进行反应,水浴10 min,用酶标仪检测并计算结果.结果 该法在1~24 mmol/L范围内线性良好,其准确性在允许偏差±5%内,变异系数(CV)＜5%,干扰实验均能满足检测的要求.对120例体检者应用本法进行血糖测定,其结果与日立7060全自动生化分析仪测定结果具有较好的相关性(r=0.987 5).结论 微板干试剂葡萄糖氧化酶法快速检测血糖结果准确可靠,检测过程简便,适合基层医疗机构快速检测及批量普查.%Objective To establish a new assay for rapid determination of blood glucose with dry reagent on a microplate reader for application in small batches of diabetes screening. Methods The improved glucose reagent was frozen and dried in the oriffice plate,and sealed with plastic film for detection by the microplate reader after adding the specimen conditioning fluid. Results The linear of the method was good within the range of 1~24 mmol/L. The accuracy was within the allowable deviation(±5%) and the repetitiveness CV was less than 5%. The interference experiment could meet the testing requirements. According to determination of blood glucose in 120 persons, the detection results by this method were well crrelated with those of Hitachi 7060 automatic biochemical analyzer(r = 0. 9875). Conclusion The rapid detection of mricoplate dry reagent glucose oxidase on blood glucose is accurate and reliable, the determination process is easy and convenient, which is suited to rapid detection of basic medical institutions and batch determination.
展开▼
