Objective To establish the model of isoprenaline(ISO)-induced chronic heart failure(CHF) in rats and to perform its evaluation. Methods 70 male SD rats were divided into four groups, normal group (n = 10),2. 0 mg · Kg-1 · D-1 group (n = 20) , 2. 5 mg · Kg-1 · D-1 group (n = 20) and 3. 0 mg · Kg-1 · D-1 group (n = 20). HF was induced by subcutaneous injections of ISO with different doses for 4 weeks. Meanwhile, the control group was established by subcutaneous injecting same volume of normal saline. Four weeks later,the rat survival rates were compared,the cardiac function tests were performed by using Doppler echocardio-graphy procedures and detected BNP,the heart weight/body weight ratio. Results The survival rates of high to low dose injection group were 30%,90% and 100%. Compared with control group, the LVEDD, LVESD, BNP and heart weight/body weight ratio were significantly increased (P<0. 01) , LVEF and FS were decreased (P<0. 05). In the control group, the more ISO was injected, the higher were LVEDD, LVESD, BNP and heart weight/body weight ratio(P<0. 01) , meanwhile the lower were LVEF,FS(P< 0. 05). Conclusion The CHF model induced by subcutaneous injecting ISO is a simple and convenient method. Although the cardie function is much worse in 3 mg · Kg-1 · D-1 ,the survival rate is also lower than other groups. 2. 5 mg · Kg-1 · D-1 ISO is a reliable dose for establishing the CHF model.%目的 建立异丙肾上腺素(ISO)诱导SD大鼠慢性心力衰竭(CHF)模型,并对其进行评估.方法 将SD雄性大鼠分为4组,ISO组每组20只均腹腔注射ISO各1次,剂量分别为2、2.5、3 mg·kg-1·d-1,建立大鼠CHF模型,并用等量生理盐水制备对照组(n＝10),分别在用药后4周进行心脏超声检测左室舒张末内径(LVEDD)、左室收缩末内径(LVESD)、左室射血分数(LVEF)、左室缩短分数(LVFS),4周末测血清B型钠尿肽(BNP)值、计算心脏质量指数.结果 注射剂量为3.0 mg·kg-1·d-1组4周末大鼠死亡14只,注射剂量为2.0 mg·kg-1·d-1无大鼠死亡,2.5 mg·kg-1·d-1组4周末大鼠死亡2只,大、中、小剂量组存活率分别为30%、90%及100%.ISO组LVEDD、LVESD、BNP及心脏质量指数均高于对照组(P<0.01),LVEF、FS低于对照组(P<0.01).ISO组LVEDD、LVESD、BNP及心脏质量指数随着ISO剂量的增加而增大,组间比较差异有统计学意义(P<0.01),LVEF、FS随着ISO剂量的增加而降低,组间比较差异有统计学意义(P<0.05).结论 皮下注射ISO诱导建立大鼠CHF模型的方法简便、可靠;心衰模型的成功建立以ISO皮下注射剂量为2.5 mg·kg-1·d-1较为适宜.
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