细胞色素P4503A5基因多态性与原发性高血压的相关性

摘要

目的：研究细胞色素 P4503A5（CYP3A5）基因多态性与中国汉族人原发性高血压（EH）的相关性。方法：采用TaqmanMGB探针建立了CYP3A5*3（6986A＞G）位点的实时荧光定量聚合酶链反应（RT-PCR）分型方法，对170例中国汉族EH患者和193例排除EH诊断的对照者进行CYP3A5*3（6986A＞G）位点基因分型。结果 EH组GG、GA、AA基因型频率分别为51．2％、42．4％、6．5％，而对照组则为39．9％、50．8％、9．3％。携带GG基因型者EH的患病风险是至少携带一个A等位基因者的1．579倍[95％ CI：1．041～2．395]。结论： CYP3A5*3（6986A＞G）多态性位点可能与中国汉族人EH具有相关性，A等位基因可能降低EH的患病风险，存在稍低的收缩压水平。%Objective To study the correlation between the cytochrome P450 3A5 gene polymorphism and essential hypertension (EH) in Chinese population .Methods The real-time PCR genotyping at CYP3A5*3(6986A>G) position was established using Taqman minor groove binding (MGB) probes .Total 170 EH patients and 193 matched controls of Chinese Han population were genotyped at CYP3A5*3(6986A>G) position using this method .Results The GG ,GA ,AA genotyped frequencies were 51 .2% , 42 .4% and 6 .5% for the EH patients and 39 .9% ,50 .8% and 9 .3% for the control group respectively .The risk of EH for person carrying GG genotype was 1 .579 fold of the persons carrying at least one A allele(95% CI:1 .041-2 .395) .Conclusion CYP3A5*3(6986A>G) polymorphism may be associated with EH in Chinese population .The risk of EH is decreased in the persons carrying allele A ,slightly lower levels of systolic blood pressure exists .