Objective To investigate the effect of adding recombinant human bone morphogenetic protein-2 (rhBMP-2 ) on the bone regeneration in sutural supramaxillary distraction osteogenesis (elasticity traction force> 800 g) .Methods 36 healthy hybrid dogs were randomly divided into 5 groups ,control ,400 g ,600 g ,800 g and 800 g+rhBMP-2 (combined) groups ,and killed in postoperative 1-8 weeks for X-ray examination and immunohistology staining .Results Supramaxilla was successfully moved forward in the various traction groups especially in the 600 g group(25.3±5 .2)mm .The traction speed in the 800 g group was fast within 2 weeks and significantly slowed after 4 weeks(12 .1±2 .3)mm ,which was improved after injection of rhBMP-2[(24 .2±3 .4)mm] . Immunohistochemical staining showed that the positive staining of BM P -2 ,BM P-4 and BM P-7 was evident in osteoblast plasma .After injection of rhBMP-2 ,the light density value in BMP-7 was obviously enhanced .The CT examinations showed that the bone density values in jawbone horizontal plate was significantly decreased especially in the 800 g group[(317 .8±185 .5)HU],but after injection of rhBMP-2 ,which was significantly enhanced[(1 158 .0±93 .0)HU ].Conclusion Sutural traction for advancing supramaxilla can extend the bone length at the early stage of microtrauma .RhBMP-2 may play certain role in the bone formation and the regulation of the activity of BMP-7 .%目的 探索上颌骨经缝牵引中(当弹性牵引力大于800 g)加入重组人骨形成蛋白-2(rhBMP-2)对新骨形成的影响.方法 将36只健康杂种犬随机分为5个组,即对照组、400 g组、600 g组、800 g组及800 g+ rhBMP-2组(联合组).术后1~8周处死动物,行X线检测及免疫组织化学染色检测.结果 各牵引组上颌骨成功前徙,以600 g组效果明显[(25.3±5.2)mm].800 g组在2周内牵引速度快,4周后明显减慢[(12.1±2.3)mm],但在注射rhBMP-2后得以改善[(24.2±3.4)mm].BMP-2、BMP-4、BMP-7阳性染色主要定位于成骨细胞细胞质中,BMP-7在注射rhBMP-2后光密度值明显增强.CT检测各牵引组腭骨水平板骨密度值明显减低,以800 g组最为显著[(317.8±185.5)HU],而在注射rhBMP-2后骨密度值明显增强[(1 158.0±93.0)HU].结论 骨缝牵引前移上颌骨能在微创早期延长骨长度;rhBMP-2可以有效促进骨形成并在调节rhBMP-7活性方面起到一定作用.
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