Objective To study the mechanism of kayadiol as one of diterpenoid compounds for inhibiting the proliferation of hu -man endometrial carcinoma cell line-l(HEC-l) .Methods The MTT assay was adopted to detect the Kayadiol' s inhibition to cell line HEC-1 proliferation .The apoptosis rate of HEC-1 induced by Kayadiol was measured by Western blot and TUNEL method . Results Kayadiol could strongly inhibit the proliferation of HEC-1 cells with dose-dependent relationship .10 μmol/L Kayadiol could induce apoptosis of HEC-1 cells with time-dependent relationship .Expression of Bax and caspase-3 proteins was significantly upregulated in HEC-1 cells treated by Kayadiol .Conclusion Kayadiol could inhibit proliferation of HEC-1 cells via inducing apoptosis of HEC-1 cells.%目的 探讨二萜类化合物kayadiol抑制子宫内膜癌细胞HEC-1增殖作用的机制.方法 采用四甲基偶氮唑盐(MTT)法检测kayadiol对子宫内膜癌HEC-1细胞增殖的抑制作用;采用DNA断裂原位末端标记(TUNEL)法和Western blot法检测kayadiol诱导子宫内膜癌HEC-1细胞凋亡.结果 kayadiol对HEC-1细胞增殖显示较强的抑制活性,呈量效关系;其中以10 μmol/L的kayadiol处理HEC-1细胞后,发现凋亡率变化与时间呈依赖关系,HEC-1细胞内的Bax和半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)蛋白表达显著上调.结论 Kayadiol可通过诱导细胞凋亡抑制HEC-1细胞增殖.
展开▼
