紫杉醇对SHI-1细胞株增殖抑制及凋亡的影响

摘要

Objective To observe the effects of taxol on appotosis rate and cell cycle of novel human monocytic leukemia cell line SHI 1 cells in vitro. Methods SHI 1 cells were treated with different concentrations of taxol for 0,12,24,36,48 h. The cell counts, trypan blue staining,DNA content determination and Annexin V/PI were used to analyze cell apoptosis and the JC 1 staining was used to detect the mitochondrial transmembrane potential. Results Taxol could inhibit the SHI 1 cells proliferation activity,show ing the dose effect relationship between the action time and dose. The typical cell morphology changes occurred,and the inhibition effect was more obvious with the drug concentration increase and the reaction time prolongation. Taxol affected the cell apoptosis rate and the cell cycle. The cells characteristic changes of apoptosis occurred after Annexin V/PI staining. The apoptotic rate and the proportion of G2/M phase were increased with the increase in drug concentration and the action time. Taxol could significantly decrease the mitochondrial transmenbrane potential. The 0. 05 mg/L treatment group had the most significant effect in inducing ap optosis and causing proliferation inhibition of SHI 1 cells. Conclusion Taxol can inhibit the SHI 1 cells proliferation activity,inducerntheir apoptosis and make them remain in the G2/M phase.%目的 研究紫杉醇对人急性单核细胞白血病细胞株SHI-1细胞凋亡率和细胞周期的影响.方法用不同浓度的紫杉醇作用于SHI-1细胞,分别作用0、12、24、36、48 h,用细胞计数、台盼蓝染色观察细胞形态学改变、DNA含量测定及异硫氰酸荧光黄(annexin-V-FITC)、碘化丙啶(PI)分析细胞凋亡、JC-1染色检测细胞线粒体跨膜电位.结果 紫杉醇能抑制SHI-1细胞增殖活性,呈现作用时间和剂量的量效关系,出现典型的细胞形态的改变,且随着药物浓度的增加和作用时间的延长,抑制作用越明显;紫杉醇对细胞凋亡率和细胞周期均有影响,AnnexinV/PI荧光染色后细胞发生凋亡的特征性改变,随药物浓度增加和作用时间的延长,细胞凋亡率增加,G2/M期比例增高;紫杉醇能使线粒体跨膜电位显著下降;0.05 mg/L处理组对SHI-1细胞株增殖抑制及诱导凋亡作用最为显著.结论紫杉醇能抑制SHI-1细胞增殖活性,诱导其凋亡,使SHI-1细胞停留在G2/M期.