单核细胞趋化蛋白-1基因-2518G/A多态性与2型糖尿病肾病的相关性研究

摘要

目的 探讨广西汉族人群单核细胞趋化蛋白-1基因-2518G/A(MCP-1-2518G/A)多态性与糖尿病肾病(DN)的关系.方法 将130例2型糖尿病患者按DN诊断标准分为DN组与糖尿病非肾病(T2DM)组,另选健康体检者80例作为健康对照组(NC组).应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术分析各组基因型及等位基因频率的分布.结果 DN组的收缩压(SBP)、舒张压(DBP)、尿微量清蛋白排泄率(UAER)、肌酐(Cr)、尿素氮(BUN)值均高于NC组及T2DM组(P＜0.05).DN 组与T2DM 组、NC 组之间基因型分布差异有统计学意义(P＜0.01),DN 组患者GG基因型频率和G等位基因频率均显著高于NC组和T2DM组(P＜0.01,P＜0.05).与NC组相比,携带G等位基因者发生DN的相对风险度增加1.406倍(P＜0.01,95%CI:1.112～1.777),携带G等位基因的DN患者血清三酰甘油(TG)、总胆固醇(TC)及低密度脂蛋白胆固醇(LDL-C)水平显著高于不携带者(P＜0.05).结论 MCP-1-2518G/A多态性与广西地区汉族人群DN的发病存在相关性,G等位基因可能是DN发病的遗传易感基因.%Objective To explore the relationship between monocyte chemoattractant protein-1 gene -2518G/A polymorphism and diabetic nephropathy (DN) in people of Han group in Guangxi. Methods One hundred and thirty patients with type 2 diabetes mellitus were divided into diabetic nephropathy (DN) group and diabetes without diabetic nephropathy (T2DM) group. Eighty healthy people were selected as normal control(NC) group. The genotypes and allele frequencies distribution were assessed by Poly-merase chain reaction-restriction fragment length Polymorphism (PCR-RFLP). Results The values of systolic pressure blood (SBP) ,diastolic blood pressure (DBP) ,urine albumin excretion rate (UAKR) , creatinine (Cr) , and blood urea nitrogen (BUN) in DN group were significantly higher than those of NC or T2DM group (P＜0.05). There was significant difference in the distribution of genotypes and allele frequencies between DN group and NC or T2DM group (P＜0.01). The GG genotypes and G allele frequency distributions in DN group were significantly higher than the NC group and T2DM group (P＜0.01 ,P＜0.05). The G allele carriers had 1. 406 times larger risk for increased incidence of DN compared with healthy controls (P＜0.01, 95%CI:1.112 -1.777). The values of TG,TC and LDL-C were significantly higher among carriers of G allele compared with non-carriers(P＜0. 05). Conclusion The polymorphism of MCP-1-2518G/A is associated with the development of DN in Han nationality in Guangxi province. G allele may be a genetic risk factor incidence of DN.