育真熄风汤对帕金森病模型小鼠脑内铁蛋白 Fn的影响

摘要

目的：探讨育真熄风汤（YZXFT ）对小鼠帕金森病（PD）模型中脑铁蛋白Fn的影响。方法将试验动物分为空白对照组、模型对照组、阳性药物对照组和中药治疗组，在1‐甲基‐4‐苯基‐1，20，3，6‐四氢吡啶（MPTP）诱导的PD小鼠模型基础上，采用免疫组织化学染色，观察各组小鼠脑内Fn蛋白的表达情况。结果爬杆实验结果显示，中药治疗组小鼠与模型对照组小鼠比较，运动协调障碍有所减轻；免疫组织化学结果显示，第6、13、20天模型对照组 Fn水平与空白对照组比较，差异有统计学意义（ P＜0．05），分别为2．21倍、1．15倍、0．36倍。YZXFT 71．5 g/kg和143 g/kg组与模型对照组比较能明显增加Fn蛋白水平。结论 YZXFT具有提高脑内Fn蛋白表达作用，为进一步研究其治疗PD的机制提供依据。%Objective To study the effect of Yuzhenxifeng Decoction on the brain ferritin in PD mouse models .Methods All ex‐perimental animals were devided into 4 groups:the control group ,the model group ,the positive drug group and TCM group .Prepare PD model mice with MPTP ,then use the immunohistochemical staining technique to observe the change of expression of Fn .Results the results showed that compared with the model group ,movement coordination disorder of rats in TCM group were relieved ;the Fn level of the model group on 6th ,13th ,20th were higher than control group(P<0 .05) ,and were 2 .21 times ,1 .15 times and 0 .36 times higher respectively .Compared with model group mice ,expression of Fn were enhanced in the treatment group .Conclusion Yuzhenxifeng Decoction can improve the expression of Fn in the brain ,which provide the basis for further study on mechanism of the treatment of Parkinson′s disease .