鼻腔鼻窦鳞状细胞癌细胞中PRL-3表达的临床意义

摘要

Objective To investigate the clinical significance of PRL-3 expression in sinonasal squamous cell cancer(SNSCC).Meth-ods The immunohistochemical method and RT-PCR were adopted to detect the PRL-3 protein expression level in 62 cases of SNSCC tissue (SNSCC group),30 cases of nasal polyps(NP group)tissue and 25 cases of normal nasal mucosa tissue(control group).The obtained results were compared.Results Both in the protein level and gene level detection,the expression of PRL-3 in the SNSCC group was higher than that in the NP group and control group,the difference was statistically significant (P <0.05).The expression of PRL-3 had no significant differences among different ages and between different genders(P >0.05),but with TNM stage increasing,differentiation degree decreasing and complicating lymph node metastasis,the expression of PRL-3 was significantly increased(P <0.05).Conclusion The PRL-3 expression can serve as good reference for the proliferation activity of SNSCC,its expressing intensity can obviously reflect the SNSCC cell proliferation activity,PRL-3 probably is an independent prognostic index of SNSCC,indicating poor prognosis.%目的：探讨促肝细胞再生磷酸酶-3（PRL-3）在鼻腔鼻窦鳞状细胞癌（SNSCC）中表达的临床意义。方法采用免疫组织化学及逆转录 PCR（RT-PCR）方法检测62例 SNSCC 组织（SNSCC 组）中 PRL-3蛋白的表达水平，并对30例鼻息肉患者（NP 组），以及25例正常鼻腔黏膜（对照组）做相同蛋白表达并进行对比。结果在蛋白水平和基因水平检测，均发现 SNSCC 组中 PRL-3的表达高于 NP 组及对照组，差异有统计学意义（P ＜0．05）。PRL-3的表达情况在不同的性别、年龄中差异无统计学意义（P ＞0．05），而随着 TNM 分期的增高，分化程度的降低及合并淋巴结转移，PRL-3的表达明显增高（P ＜0．05）。结论 PRL-3的表达可以对SNSCC 的增殖活性作很好的参照，表达强度可明显反映 SNSCC 细胞增殖活性，PRL-3可能是 SNSCC 的一个独立预后指标，提示预后不良。