藏药佐太中汞在小鼠体内的蓄积

摘要

目的 探索给予KM小鼠不同剂量和不同时间的佐太后,汞在体内的蓄积情况.方法 KM小鼠随机分为空白组,佐太低、中、高剂量组(42 d,6.07、60.70、606.97 mg/kg;14 d,606.97 mg/kg),给药后,测定小鼠脑(嗅球、皮层、海马、下丘脑、脑干、小脑)、心脏、肺脏、肾脏、肝脏、脾脏、血清、肌肉中汞含有量.结果 与空白组相比,给予42 d低剂量佐太后,能够显著增加小鼠海马、小脑、肺脏、肾脏、肝脏、血清中汞含有量;给予42 d中剂量佐太后,能够显著增加小鼠嗅球、皮层、海马、脑干、小脑、心脏、肺脏、肾脏、肝脏、脾脏、血清中汞含有量;给予42、14 d高剂量佐太后,能够显著增加小鼠嗅球、皮层、海马、下丘脑、脑干、小脑、心脏、肺脏、肾脏、肝脏、脾脏、肌肉、血清中汞含有量.结论 小鼠灌胃佐太后,汞在不同组织中产生蓄积,且表现出剂量和时间依赖性,提示佐太及其复方制剂不宜过量、长期使用.%AIM To explore the mercury accumulation in KM mice after being given Zuotai at different doses and time.METHODS KM mice were randomly divided into blank group,Zuotai low-,middle-and high-dose (6.07,60.70 and 606.97 mg/kg,42 d;606.97 mg/kg,14 d) groups.The mercury contents in brain (olfactory bulb,cortex,hippocampus,hypothalamus,brain stem,cerebellum),heart,lung,kidney,liver,spleen,serum,muscle of mice were measured after administration.RESULTS Compared with the blank group,Zuotai at low-dose significantly increased the mercury contents in hippocampus,cerebellum,lung,kidney,liver and serum of mice after 42-day treatment;Zuotai at middle-dose markedly increased the mercury contents in olfactory bulb,cortex,hippocampus,brain stem,cerebellum,heart,lung,kidney,liver,spleen and serum of mice after 42-day treatment;the mice treated with high-dose of Zuotai for 42,14 days significantly increased the mercury contents in olfactory bulb,cortex,hippocampus,hypothalamus,brain stem,cerebellum,heart,lung,kidney,liver,spleen,muscle and serum.CONCLUSION Mercury can be accumulated in different tissues of mice after intragastric administration of Zuotai in a dose-and time-dependent manner,which suggests that Zuotai and its compound preparations should not be used in high-dose and long-term.