目的 测定藤茶Ampelopsis grossedentata(Hand.-Mazz.)W.T.Wang不同繁殖方式有效成分,分析其抗氧化酶活性.方法 采用单因素及正交试验筛选抗氧化酶的最佳提取工艺,测定普通扦插、两段式扦插和组织培养藤茶叶片的抗氧化酶活性;采用溶剂萃取法提取3种繁殖方式中藤茶茎叶的有效成分并测定其含有量.结果 藤茶3种繁殖方式中,组培苗多糖含有量最高,两段式扦插苗黄酮和多酚含有量最高;抗氧化酶提取最优工艺为提取液pH6.0,处理温度20℃,处理时间20 min;抗氧化酶活性依次为两段式扦插苗＞普通扦插苗＞组培苗.结论 考虑到藤茶有效成分含有量和抗氧化酶活性,3种繁殖方法中两段式扦插法最佳.%AIM To determine effective components of Ampelopsis grossedentata (Hand.-Mazz.) W.T.Wang under different breeding modes and to analyze the antioxidant enzyme activities.METHODS Single factor and orthogonal tests were adopted in screening the optimal extraction process of antioxidant enzymes and analyzing antioxidant enzyme activities of A.grossedentata under common cutting,two-phase cutting and tissue culture.Solvent extraction method was adopted in extracting the effective components of three breeding modes in stem leaves of A.grossedentata,whose contents were determined.RESULTS In three breeding modes,the content of polysaccharides in tissue culture seedling was the highest,while the contents of flavonoids and polyphenols in the two stage cutting seedlings were the highest.The optimal extraction process of antioxidant enzymes was determined to be 6.0 for pH,20 ℃ for processing temperature and 20 min for processing time.Antioxidant enzyme activities were in sequence of two stage cutting seedlings ＞ common cutting seedling ＞ tissue culture seedling.CONCLUSION Considering the contents of effective components and antioxidant enzyme activities,two stage cutting method is the best among 3 breeding modes.
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