坏损外担菌培养液对烤烟幼苗生长及主要生理特性的影响

摘要

为探明坏损外担菌培养液对烤烟幼苗生长和生理特性的影响,研究了坏损外担菌培养液不同浓度(原液、1/10原液、1/50原液和1/100原液)对烤烟幼苗叶片、茎和根系生长及叶片叶绿体色素、NR活性和根系活力等的影响.结果表明,处理5 d时,1/50原液最大叶长和根系鲜质量最大,与原液达到显著差异,1/50原液茎高、地上部鲜质量和叶绿素b最大,显著高于其他处理和对照.处理10 d时,1/50原液最大叶长、最大叶宽、叶面积、茎高、茎围、叶绿素a和类胡萝卜素含量最高,且显著高于原液、1/100原液和对照,1/50原液NR活性和根系活力最高,且与原液和对照达到显著水平.处理15 d时,1/50原液最大叶长、最大叶宽、叶面积、茎高、地上部鲜质量、NR活性和根系活力最大,显著大于其他各处理和对照,而茎围、根鲜质量和叶绿素a含量显著大于原液、1/100原液和对照.坏损外担菌培养液不同浓度对烤烟幼苗生长和生理代谢影响不同,1/50原液烟苗生长最好.%To investigate the effects of Exobasidium vexans liquid culture on the growth and physiological characteristics of tobacco seedlings, experiments were carried out to study the effects of different concentrations [the original solution, 1/10 original solution, 1/50 original solution,1/100 original solution]of E. vexans liquid culture on growth of leaves, stems and roots, chloroplast pigment, nitrate reductase (NR) activity and root activity of tobacco seedlings. On the 5th day, the maximum leaf length and root fresh mass of the 1/50 original solution were significantly different from that of the original solution, and the stem height, shoot fresh mass and content of Chl b were significantly higher than those of other treatments and the control. On the 10th day, the maximum leaf length and width, leaf area, stem height, stem circumference, chlorophyll a and carotenoid content of 1/50 original solution were the highest with significant difference from the original solution, 1/100 original solution and the control. The NR activity and root activity of 1/50 original solution were the highest and significantly higher than the original solution and the control. On the 15th day, the maximum leaf length and width, leaf area, stem height, shoot fresh mass, NR activity and root activity of 1/50 original solution were the highest with significant difference from the other treatments and the control. But the stem circumference, root fresh mass and chlorophyll a content of 1/50 original solution were significantly higher than that of the original solution, 1/100 original solution and control. The effects of different concentrations of E. vexans liquid culture were different on the growth and physiological characteristics of tobacco seedlings. The growth of tobacco seedlings was the best in 1/50 original solution.