湖北地区烟草青枯菌系统发育分析

摘要

For further details physiological specialization of Ralstonia solanacearum strains isolated from Hubei Province, 48 R.solanacearum strains of tobacco were analyzed using the phylotype classification schemes. Phylogenetic analysis of endoglucanase gene(egl) and DNA mismatch repair protein gene(mutS) sequences showed that all isolates belonged to phylotypeⅠ, including 3 different sequevars 15, 17 and 44. 19 strains were belonged to sequevar 17 and 26 strains were belonged to sequevar 44, Only 3 strains were identified as sequevar 15.R.solanacearum strains of tobacco from Hubei Province had genentic differentiation of sequevar level.%本研究采用青枯菌演化型分类框架，对来源于湖北烟区的48个烟草青枯菌进行系统发育学分析并进行序列变种鉴定，以明确该地区烟草青枯菌的菌系分化。基于青枯菌内切葡聚糖酶基因（egl）和DNA蛋白修复基因（mutS）的系统发育学分析结果表明，48个供试菌株属于青枯菌亚洲分支（演化型Ⅰ）的3个序列变种，分别为序列变种15、17和44，未发现我国已报道的烟草青枯菌序列变种34。其中序列变种17和44为优势菌系，且均来源于恩施地区；序列变种15只包含来源于十堰地区的3个菌株。本研究表明湖北地区烟草上的青枯菌存在一定程度的遗传分化。