外周血CD19+CD23+B细胞在不同严重程度哮喘患儿中的表达

摘要

Objective To investigate the expression and significance of CD19+CD23+B cells in children with mild, moderate and severe asthma. Methods Fifty-six patients with asthma episodes were enrolled from the in-patient and out-patient department of Shanxi Women and Children's Hospital between February 2011 and February 2012, and graded for severity of asthma episodes as severe (group A), moderate (group B) and mild (group C). The 36 normal controls were recruited from Shanxi Epidemiological Survey of Prevalence of Asthma. Flow cytometry was employed to detect the percentage of CD19+CD23+B cells in peripheral blood and fluorescence enzyme-linked im-munosorbent assay to measure the total serum immunoglobin E (IgE). Results Children with asthma episodes, showing the highest percentage of CD19+CD23+B cells in peripheral blood in group A, followed by groups B and C (P＜ 0.05), presented a significantly higher percentage than control controls (P＜0.05). However, the difference in groups C and normal controls did not achieve statistical significance (P＞0.05). The level of total serum IgE was highest in group A, followed by groups B, C and normal controls (P＜0.05). The percentage of CD19+CD23+B cells correlated positively with total serum IgE (r=0.538, P＜0.05). Conclusion Children with asthma are characterized by increased peripheral blood CD19+CD23+B cell count, which increases progressively with the severity of episodes. The combination of posi-tivity of peripheral blood CD19+CD23+B cells and total serum IgE level may be the surrogate for assessment of asthma.%目的 探讨哮喘轻、中、重度急性发作患儿外周血CD19+CD23+B细胞的表达与意义.方法 收集2011年2月至2012年2月山西省儿童医院门诊及住院哮喘急性发作患者56例为试验组,依据哮喘急性发作程度分级标准,根据其发病情况,将其分为重度(A组)、中度(B组)、轻度组(C组)；并收集参与山西省哮喘发病率流行病学调查的健康儿童36名作为对照组,采用流式细胞技术检测其外周血CD19+CD23+B细胞比率,采用酶联免疫吸附法(ELISA)检测其血清总IgE水平.结果 ①哮喘急性发作患者外周血CD19+CD23+B细胞水平高于健康儿童,同时,A组＞B组＞C组,差异有统计学意义(P＜0.05)；且A组与B组均高于健康对照组(P＜0.05)；C组高于健康对照组,但差异无统计学意义(P＞0.05)；②血清总IgE水平A组＞B组＞C组＞健康对照组,差异有统计学意义(P＜0.05).③CD 19+CD23+B细胞与总IgE水平呈正相关(r=0.538,P＜0.05).结论 哮喘患者外周血CD19+CD23+B细胞升高,并随病情加重而升高.外周血CD19+CD23+B细胞阳性表达率联合血清总IgE水平可用作哮喘病情评估的辅助手段.