寡核苷酸促人牙周膜细胞成骨向分化机制的实验研究

摘要

Objective To investigate the effect of oligodeoxynucleotide mt01 (ODN mt01) on the osteogenic differentiation of human periodontal ligament cells (hPDLCs), and to provide an experimental basis for the develop-ment of clinical agents. Methods The in vitro hPDLCs were included as the subjects in the study for primary culture and subculture. The third generation of hPDLCs and ODN mt01 with the working concentration of 1.0 mg/L were co-cultured for 24 h, 48 h, 72 h and 96 h. The effect of ODN mt01 on the osteogenic differentiation of hPDLCs was de-termined by real time PCR. Results At 24 h, 48 h, 72 h after the co-culture, ODN mt01 with the working concentra-tion of 1.0 mg/L significantly up-regulated the mRNA expression levels of the osteogenic factors Runx-2, Sp7 and Col-lagen-I in hPDLCs, respectively, compared with those in the PBS control group ( P<0.05). Conclusion The appropri-ate working concentration of ODN mt01 may increase the gene expression levels of the osteogenic factors to promote the osteogenic differentiation of hPDLCs.%目的：研究寡核苷酸（ODN）mt01对人牙周膜细胞向成骨细胞分化的影响，为临床药剂的开发奠定实验基础。方法采用人离体牙牙周膜细胞作为研究对象，原代培养牙周膜细胞并传代，取第三代人牙周膜细胞，加入工作浓度为1.0 mg/L的ODN mt01共培养24 h、48 h、72 h和96 h后，通过实时聚合酶链反应（real time PCR）法研究ODN mt01对人牙周膜细胞成骨分化的影响。结果分别作用于人牙周膜细胞24 h、48 h、72 h和96 h后，与磷酸盐缓冲液（PBS）对照组相比，工作浓度为1.0 mg/L的ODN mt01可显著上调人牙周膜细胞中Runx-2、Sp7和Ⅱ型胶原等成骨相关因子mRNA的表达水平（P＜0.05）。结论适当工作浓度的ODN mt01可能通过增加成骨相关因子的基因表达水平进而促进人牙周膜细胞的成骨向分化。