Aim:Method for the deternination of erythromycin ethylsuccinale(EES) by ion suppression chromatography(ISC) was developed and the influenc factors on ISC were investigated.Methods:A Zorbax SB-C18 column was used with 0.02mol.L^-1 potassium dihydrogen phosphate-acetonitrile(45:55) as mobile phase,The pH and proportion of the mobile phase showed the greatest influences on retention and selectivity.Therefore,the pH of mobile was adjusted to 6.8,the bhest acetonitrile proportion was 55%.The column temperature was maintained at (300±0.5)℃.Acetonitrile was used as solvent for the sample preparation because EES is more stable in it.The flow rate was 1.2mL.min^-1 and UV detection was performed at 210nm.Results :Under these chromatographic conditions,the main component (erythromycin A ethylsuccinate)and its related substances were separated.The calibration curve showed good linearity over the range of 0.1-1.0mg.mL^-1,and its correlation coefficient was 0.9998.Conclusion:The method is very suitable for the analysis of erythromycin ethylsuccinate.
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