The study of Gongshisong＇ s anti-fatigue active site sifting

摘要

Aim To investigate the influence of the Gongshisong extract of different extraction parts on mice sports fatigue index. Method The mice were randomly divided into blank group, model group, the Gongshisong extract group （petroleum ether, chloroform, ethyl acetate, n-butanol, the aqueous layer） and the positive control group. The mice in different groups were fed with corresponding Gongshisong extract liquid 2.5 g kg^-1 d^-1 once per day for consecutively 7 days. The blank group and model group were given 1% sodium carboxymethyl cellulose so- lution, the positive control group was administered with Rhodiola 0.59 g · kg^-1· d^-1 After the last administered each group of mice were determined of weihgt-loading swimming time, liver glycogen and muscle glycogen content, and urea nitrogen, lactic acid and creatine kinase in 90min mice after swimming content in blood. Result Com- pared with the blank group, the petroleum ether extract of Gongshisong could significantly prolong the swimming time of mice （P 〈 0.05）, enhancing the mice muscle glycogen reserves （P 〈 0.05）, and reduce the level of urea nitrogen and creatine kinase in mice after swimming （P 〈 0.05）. Butanol extraction liquid of Gongshisong can ob- viously prolong the swimming time of mice （P 〈 0.05）, increasing the reserves of glycogen in mice （P 〈 0.05）, decreasing the level of urea nitrogen in mice and blood lactate after swimming （P 〈 0.05 ）. Water extract of Gong- shisong could significantly prolong the swimming time of mice （P 〈 0.05） , increasing muscle glycogen storage （P 〈0.05）, reducing the level of blood lactic acid in mice after swimming （P 〈 0.05）. Ethyl acetate extract of Gongshisong could significantly prolong the swimming time of mice （P 〈 0.05） , and increase the reserves of glyco- gen in mice （P 〈0. 05）. Conclusion Comprehensive analysis, the petroleum ether, n-butanol and water site of Gongshisong is better anti fatigue active site.