补阳还五汤精简方对大脑中动脉阻塞模型大鼠海马组织Cdk5的调控

摘要

Aim To evaluate the regulation of thin recipe of Buyang Huanwu decoction on cyclin-dependent kinase 5(Cdk5)expressions in hippocampus tissue of rats after cerebral ischemia.Methods Male SD rats were divided into sham-operation group,MCAO group,Buyang Huanwu decoction group(ig.3.15 g·kg-1)and its thin recipe composition group(ig.2.41 g·kg-1).Each group was then divided into five subgroups based on the time after administration for 1,3,7,14,28 d respectively.Cdk5 protein and mRNA levels in each group were examined by using immunohistochemistry,Western blot and real-time PCR respectively.Results The up-regulation of Cdk5 was observed in model rat hippocampus after cerebral ischemia 1 day,and kept increasing with the aggravation of ischemia injury,the peaked expression was observed after 7～14 d,while the downtrend was observed after 28 days compared with the corresponding sham-operation groups(P0.05).Conclusion The thin recipe of Buyang Huanwu decoction could exert the protective effect by regulating Cdk5 after cerebral ischemia.%目的 评价补阳还五汤精简方对大脑中动脉阻塞(MCAO)模型大鼠海马组织周期素依赖性蛋白激酶5(cyclin-dependent kinase 5,Cdk5)的调控.方法 将♂ SD大鼠随机分为假手术组、MCAO模型组、补阳还五汤干预组(灌胃3.15 g·kg-1)及补阳还五汤精简方干预组(灌胃2.41 g·kg-1),每组按给药后1、3、7、14、28 d再各分5小组.采用免疫组化法、Western blot法和RT-PCR法考察不同时间点各组大鼠海马组织中Cdk5的表达.结果 MCAO模型大鼠海马组织中,脑缺血后1 d即出现Cdk5上调趋势,并随着脑缺血的加剧,Cdk5的表达继续上调,其中mRNA及蛋白水平在7～14 d达最大,28 d则出现下降趋势,与各时间点假手术组比较P0.05).结论 补阳还五汤精简方可能通过调控Cdk5的表达水平对脑缺血海马组织发挥保护作用.