普洱茶提取物的抗HIV活性

摘要

AIM:To evaluate the anti-HIV activities of extracts from two commonly forms of Pu-erh tea (fermented),green tea-like (unfermented).METHODS:The cytotoxicities of extracts on C8166,MT4 and PBMC were assessed by MTT and the anti-HIV activities of extracts were evaluated by syncytium reduction and p24 antigen assay.RESULTS:All extracts had low cytotoxicities with the CC50 from 120.63 to 524.95 μg·mL-1.All extracts can inhibit HIV-induced cytopathic effects,with EC50 various from 11.13 to 67.49 μg·mL-1.The WEPT's were better than EEPTs,moreover the FPT's water extracts were better than the GTLPT's especially YYP-31 with SI value 42.40.YYP-31 also inhibited HIV-1RF and HIV-2CBL-20 infection with EC50 of 30.82 and 39.79 μg·mL-1,respectively.YYP-31 reduced p24 antigen expression in HIV-1ⅢB acute infected C8166 and in HIV-1KM018 infected PBMC with EC50 values of 14.95 and 74.63 μg·mL-1,respectively.YYP-31 blocked the fusion between normal C8166 cells and HIV-1 chronically infected H9 cells with EC50 values of 234.27 μtg·mL-1.It exhibits markedly synergistic anti-HIV activity in combination with AZT but can't inhibit activities of reverse transcriptase.CONCLUSION:Pu-erh tea extracts exhibit highly efficient anti-HIV-1 activity and can be considered to be used to as a complementary therapy or a health product to HIV patients.%目的:研究普洱熟茶(发酵)和普洱绿茶(未发酵)这2种常见普洱茶的抗HIV作用.方法:采用MTT比色法检测普洱茶提取物对C8166,MT4和PBMC细胞的毒性作用；细胞病变法检测化合物对HIV-1急性感染的抑制活性.采用细胞病变法和HIV-1 p24抗原ELISA方法检测普洱茶提取物的抗HIV活性作用；结果:所有普洱茶提取物都具有较低的细胞毒性CC50为120.63-524.95μg·mL-1.所有普洱茶提取物均能抑制HIV诱导细胞形成合胞体其EC50为11.13-67.49μg·mL-1.普洱茶水提物的抑制作用比醇提物的抑制作用要好,并且普洱熟茶的抑制作用好于普洱生荼,尤其是YYP-31,其SI值为42.40.YYP-31对HIV-RF和HIV-2CBL20也有很好的抑制作用,其EC50分别为30,82和39.79 μg·mL-1.YYP-31还能很好地抑制HIV-1ⅢB急性感染C8166细胞和HIV-1KM018感染PBMC细胞p24抗原的产生,其EC50分别为14.95和74.63 μg·mL-1.YYP-31能阻止正常细胞C8166与HIV-1慢性感染细胞H9/HIV-1ⅢB之间的融合,EC50为234.27 μg·mL-1.提取物YYP-31与AZT联合用药具有显著的协同抗HIV作用,但是对HIV逆转录没有抑制作用.结论:普洱茶提取物具有很好的抗HIV的活性作用,可以作为一种HIV辅助治疗的产品或保健品用于HIV患者的治疗过程中.