EXPRESSION OF HLA-CLASS Ⅱ GENESOF IDDM PATIENTS ON THE SURFACE OF THE LTK- CELLS

摘要

To investigate the function of HLA-class Ⅱ genes in the autoimmune response of insulin dependent diabetes mellitus(DDM),the HLA-class Ⅱ gene of IDDM patients was introd uced into Ltk^-cells with pSV2-neo plas-mid,using the calcium phosphate precipitation technique.We obtained a stable cell line expressing the HLA-class Ⅱ gene from lymphocytes of IDDM patients.Expression was identified by direct ox erythocyte-CrCl3-HLA DR monoclonal antibody rosetting.