用原位杂交方法检测虹膜睫状体EP1和FP受体mRNA的表达

摘要

Objective To determine the expression of E-prostanoid1 (EP1) and F-prostanoid (FP) receptor mRNAs in iris-ciliary bodies of the human eye using in situ hybridization.Methods EP1 and FP receptor mRNAs were detected by riboprobes labeled with digoxigenin on paraffin sections of the iris-ciliary body tissue of the human eye using in situ hybridization. Results EP1 and FP receptor mRNAs were highly expressed in blood vessels, muscles and the endothelia of the iris. EP1 receptor hybridization signals were present in all muscle fibers of the ciliary body. Hybridization signal corresponding to FP receptor mRNA transcript was predominantly expressed in the circular muscle and in the collagenous connective tissues of the ciliary body. FP receptor mRNA was not detected in radial and longitudinal muscles. Conclusions EP1 and FP receptor mRNAs in human ocular tissues appear to be widely localized in the functional sites of the respective receptor agonists. Selective localization of EP1 and FP receptor mRNAs in the circular muscles and collagenous connective tissues of the ciliary body suggests that EP1 and FP receptors play an important role in enhancing uveoscleral outflow of aqueous humor.%目的检测人眼虹膜-睫状体组织内EP1和FP受体mRNA的表达情况.方法以地高辛标记EP1和FP受体探针,用原位杂交方法检测人眼石蜡切片虹膜-睫状体相应受体mRNA的信号表达.结果虹膜的血管、肌肉和内皮层见大量EP1和FP受体mRNA表达.EP1受体mRNA信号见于睫状体所有的肌肉纤维;FP受体杂交信号主要见于睫状体环形肌肉和结缔组织,纵行肌和放射状肌肉的信号未能检测到.结论人眼组织EP1和FP受体mRNA广泛表达于相应有关受体激动剂作用的功能部位.EP1和FP受体mRNA选择性的表达于睫状体环形肌和结缔组织,提示二种受体在增加巩膜脉络膜房水引流过程中起着重要作用.