目的 建立大鼠C6脑胶质瘤模型,寻找简易判定颅内肿瘤大小的可靠指标.方法 应用立体定向法,将1%琼脂糖含1×106 C6瘤细胞悬液10μl 注入大鼠右脑尾状核并行一般观察和MRI检查.在接种后第10、15、20、25天和自然死亡前,对大鼠做主动脉多聚甲醛灌注固定,对脑组织和肿瘤标本进行大体观察和HE染色.结果 50只接种大鼠均有颅内肿瘤生长（100%）,远处和颅外转移率为0%-4%.结论 成功建立大鼠C6脑胶质瘤模型,接种后大鼠的生存时间可作为判定颅内肿瘤生长大小的指标.%Objective To establish rat C6 brain-tumor models and find a simple reliable index to judge tumor volume. Methods C6 cell suspension (10μl) containing 10g/L agarose and 1×106 cells was injected into the right caudate nucleus of the rat brain by a stereotaxic method. After implantation, rats were observed and given MRI scans. Rats were perfused with paraformaldehyde trans-aorta at the 10th, 15th, 20th and 25th day and before natural death. All brains, lungs, spinal cords and tumors were sectioned and inspected. Tumor-containing samples were prepared histologically by hematoxylin and eosin (HE) stains. Results Fifty implanted rats had 100% yield of intracerebral growth, with distant metastasis of 0% to 4%. Conclusion A rat C6 brain-tumor model was successfully established. Rat survival time is correlated with tumor volume and may be useful as an index of tumor size.z
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