Expression of Brain-derived Neurotrophic Factor and Tyrosine Kinase B in Cerebellum of Poststroke Depression Rat Model

摘要

Background:The pathophysiology of poststroke depression (PSD) remains elusive because of its proposed multifactorial nature.Accumulating evidence suggests that brain-derived neurotrophic factor (BDNF) plays a key role in the pathophysiology of depression and PSD.And the cerebellar dysfunction may be important in the etiology of depression;it is not clear whether it also has a major effect on the risk of PSD.This study aimed to explore the expression of BDNF and high-affinity receptors tyrosine kinase B (TrkB) in the cerebellum of rats with PSD.Methods:The rat models with focal cerebral ischemic were made using a thread embolization method.PSD rat models were established with comprehensive separate breeding and unpredicted chronic mild stress (UCMS) on this basis.A normal control group,depression group,and a stroke group were used to compare with the PSD group.Thirteen rats were used in each group.Immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) for detecting the expression of BDNF and TrkB protein and mRNA in the cerebellum were used at the 29th day following the UCMS.Results:Compared with the normal control group and the stroke group,the number ofBDNF immunoreactive (IR) positive neurons was less in the PSD group (P ＜ 0.05).Furthermore,the number ofTrkB IR positive cells was significantly less in the PSD group than that in the normal control group (P ＜ 0.05).The gene expression of BDNF and TrkB in the cerebellum of PSD rats also decreased compared to the normal control group (P ＜ 0.05).Conclusions:These findings suggested a possible association between expression of BDNF and TrkB in the cerebellum and the pathogenesis of PSD.