原子力显微镜对高血压患者红细胞力学性质的测定

摘要

目的:探讨高血压患者的红细胞力学性能.方法:采集32例高血压患者的静脉血,根据血压水平(Ⅰ级、Ⅱ级和Ⅲ级)将其分为H1、H2、H3组,同时获取8例健康成年人的静脉血作为对照组,分离出血样中的红细胞,常规检验获得红细胞体积分布宽度-变异系数(red cell distribution width-variation coefficient,RDW-CV),用原子力显微镜分别检测各组红细胞的高度和弹性模量(elastic modulus,EM),并进行统计分析.结果:H2、H3组高血压患者红细胞的平均RDW-CV与对照组相比明显增大(P＜0.05,P＜0.01),H2、H3组高血压患者红细胞的平均高度明显低于对照组(P＜0.05),H2、H3组高血压患者红细胞EM明显大于对照组(P＜0.05);EM和RDW-CV存在一定相关性(R2=0.629).结论:高血压可影响红细胞的EM,原子力显微镜在单细胞水平上为红细胞的力学特征提供有效的检测.该研究从生物力学的角度探讨了高血压与红细胞结构和功能的关系.%Objective To explore the mechanical properties of erythrocytes in the patients with hypertension.Methods Venous blood of 32 patients with hypertension were collected and divided into three groups of H1,H2,and H3 (degree Ⅰ,Ⅱ,Ⅲ),with 8 cases of healthy adults into a control group.Erythrocytes were isolated,the diameter,height and elastic modulus were detected by the atomic force microscope (AFM).Statistical analysis was carried out.Results The RDW-CV of H2,H3 were much larger than the control group (P＜0.05,P＜0.01);The height of erythrocyte in H2,H3 groups were lower than that of the control group (P＜0.05).The elastic modulus of H 1,H2 groups were larger compared with normal erythrocytes (P＜0.05).Furthermore,erythrocytes elastic modulus correlated with RDW-CV (R2=0.629).Conclusion Hypertension could affect the elasticity modulus of erythrocytes.AFM could be an effective tool in measuring the mechanical characteristics of erythrocytes at single cell level.This study investigates the relationship between hypertension and the structure and function of erythrocytes from a biomechanical aspect.