首次从国内鉴定到猪肠道杯状病毒及其分子流行病学调查

摘要

目的 了解国内猪札如病毒(猪肠道杯状病毒成员)的存在和流行情况.方法 从多个腹泻猪场共采集腹泻猪粪便189份,采用RT-PCR方法进行分子流行病学调查.结果 检出猪札如病毒阳性病料24份,阳性率为12.70%(24/189).测序后与国外已发表的毒株比较,表明了所测定流行毒株的序列与猪札如病毒代表株Cowden相近(同源性介于75.6%～88.3%之间),证实了其均为猪札如病毒.结论 首次发现我国猪群中存在猪札如病毒.%A total of 189 stool samples from swine with diarrhea, collected in various porcine farms in the central region of China were tested for porcine enteric caliciviruses (PEC) member porcine sapoviruses (SaV) by reverse transcription polymerase chain reaction (RT-PCR) amplification using primers designed to detect porcine SaV. Selected amplicons were sequenced to establish phylogenetic relationships with reference strains. Porcine SaV were detected in 12.70% (24/189) of the samples. Phylogenetic studies based on partial RNA polymerase gene sequences indicated that the field strains of viruses isolated in China were closely related (75.6 88.3% identity) to the porcine SaV Cowden reference strain. These results provide evidence that caliciviruses of the genus sapovirus circulate in piglets in China, but further studies are needed to clarify their importance as cause of diarrhea. This is the first report of PEC in China.