合欢皮总皂苷治疗旋毛虫感染小鼠的疗效观察

摘要

目的 研究合欢皮总皂苷对感染旋毛虫小鼠的治疗效果.方法 将36只感染旋毛虫的ICR小鼠随机分为6组(每只小鼠感染300条旋毛虫),每组6只.组Ⅰ-Ⅲ为成虫组:组Ⅰ为感染对照组,组Ⅱ为合欢皮总皂苷治疗组,组Ⅲ为阿苯达唑治疗组;组Ⅳ-Ⅵ为肌幼虫组:组Ⅳ为感染对照组,组Ⅴ为合欢皮总皂苷治疗组,组Ⅵ为阿苯达唑治疗组.Ⅰ、Ⅱ、Ⅲ组于感染后第2d开始给药,连续给药3d,于感染后第7d处死,计数小肠内成虫;Ⅳ、Ⅴ、Ⅵ组于感染后第7d开始给药,连续给药14 d,于感染后第40 d处死,计数肌幼虫并计算减虫率,HE染色计数肌幼虫,免疫组化检测膈肌中IL-1β,IL-6,TNF-α,COX-2因子的表达.结果 合欢皮总皂苷和阿苯达唑治疗组成虫数和肌幼虫数均少于感染对照组(P＜0.01),成虫减虫率分别为71.60％和81.24％,肌幼虫减虫率分别为65.70％和89.94％;HE染色结果表明两个治疗组囊包幼虫均减少,炎症细胞表达均明显减轻;免疫组化结果显示治疗组IL-1β,IL-6,TNF-α,COX-2的表达降低.结论 合欢皮总皂苷对旋毛虫成虫和囊包幼虫均有较好的杀虫效果,虽然效果略次于阿苯达唑,但其作为中药提取物毒性较低.%We investigated the therapeutic effect of Albizia julibrissin total saponins on mice infected with Trichinella spiralis.Thirty-six ICR mice infected with Trichinella spiralis were randomly divided into 6 groups (each mouse infected with 300 T.spiralis),6 mice in each.Group Ⅰ:infected non-treated group (intestinal phase);group Ⅱ..received Albizia julibrissin total saponins group (intestinal phase);group Ⅲ:received albendazole group (intestinal phase);group Ⅳ:infected nontreated group (muscular phase);group Ⅴ:received Albizia julibrissin total saponins group (muscular phase);group Ⅵ:received albendazole group (muscular phase).Mice of Ⅰ,Ⅱ,Ⅲ group were administered on the second days post-infection(dpi) and continued for 3 days.Mice in these groups were sacrificed 7th dpi and adult worms recovered from the small intestine were counted.Mice of Ⅳ,Ⅴ,Ⅵ group were administered on the 7th dpi and continued for 14 d.The mice were sacrificed on 40th dpi,and the muscle larvae were counted.HE staining counts muscle larvae and the expression of IL-1β,IL-6,TNF-α and COX-2 in the diaphragm were detected by immunohistochemistry.Results showed that the number of adult worms and larva in groups received Albizia julibrissin total saponins and albendazole were significantly lower than that of infected non-treated group (P＜0.01).The worms reduction rate was 70.34％ and 80.02％ respectively,and the larva were 65.60％ and 90.66％ respectively.Results of HE staining showed the number of encysted larval and the expression of inflammatory cell were significantly reduced.The expression of IL-1β,IL-6,TNF-α and COX-2 was decreased in drug-treated groups.In conclusion,the total saponins of Albizia julibrissin showed adequate efficacy on Trichinella spiralis adults and encapsulated larva.Although the effect is slightly inferior to albendazole,as traditional Chinese medicine extract,it is less toxic.