在1999年3月至2000年6月，对卧龙自然保护区大熊猫研究中心的9只5.5～16.5岁的雄性大熊猫进行了17次电刺激采精，9只大熊猫的平均体重为108.1±15 kg，睾丸体积为289.4±108.6 cm3，刺激电压2～8 V，射精电压4～8 V，平均每次采精量为3.5±1.91 ml，精子密度为995.2±782.6×106个/ml，每次采精总量为3907±2981.7×106个，精液平均活力为69.1±28.9%，活率为79.6±23.1%，运动状态为2.7±1.1，畸形率为39.4±32.2%，平均顶体正常率为85.8±31.2%，pH值为7.9±0.4。其中3只是能进行自然交配并繁殖了后代的种公兽，其体重为122.7±7.1 kg，睾丸体积为381.7±64.8 cm3，精液活力为77.3±5.8%，运动状态为3.0±0.5，精子畸形率为18.0±9.6%，顶体正常率为92.7±1.5%，pH值为8.0±0.1。大熊猫异常精子主要有：顶体异常，卷尾，中段弯曲含胞浆小滴，中段弯曲无胞浆小滴，尾弯曲含胞浆小滴，尾弯曲无胞浆小滴，近端含胞浆小滴，远端含胞浆小滴8种。在2月～6月都能通过电刺激采集到精液，在4月份每次产精量最高，这正好与在这期间发情和交配的大熊猫数量最多相吻合。%Nine male pandas between 5.5 to 16.5 years old at the Research and Conservation Center for Giant Panda in Wolong Nature Reserve were used for this study.Spermatozoa were collected by electroejaculation.The average body weight of nine males was 108.1±15KG.The testes volume was 289.4±108.6cm3.Total spermatozoa per ejaculate was 3907±2981.7×106. The sperm motility was 69.1±28.9%，speed of progression was 2.7±1.1，the percent abnormal sperm count was 39.4±32.2%，normal acrosome count was 85.8±31.2%，pH was 7.9±0.4.Three of the males already have produced offspring through natural mating. The average weight of these three males was 122.7±7.1KG.Their average testes volume was 381.7±64.7cm3.Their sperm motility was 77.3±5.8%,status was 3.0±0.5,average percentage of abnormal sperm was 18±9.6%，normal acrosome was 92.7±1.5%,pH was 8.0±0.1.Abnormalities of giant pandas' spermatozoa ware including:abnormal acrosome,tightly coiled tail,bent midpiece w/droplet,bent midpiece w/o droplet,bent tail w/droplet,bent tail w/o droplet,proximal droplet,distal droplet.
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