转录因子核因子-κB p65通过miR-17调控血管平滑肌细胞增殖的机制研究

摘要

目的 研究miR-17在血管平滑肌细胞(VSCM)增殖中的作用,以及转录因子核因子(NF)-κB调控miR-17的作用机制.方法 将miR-17mimics转染人VSCM,CCK-8法检测细胞活力,流式细胞术检测细胞周期.生物信息学预测NF-κB p65与miR-17启动子区存在结合位点,将pcDNA3.1-p65与报告基因载体pGL3-miR-17启动子区共转染至293T细胞中,通过检测荧光素酶报告基因Luc活性分析NF-κB p65对miR-17启动子区的影响.脂多糖(LPS)刺激细胞后,检测NF-κB p65、miR-17的表达水平.结果 与miR-NC组相比,转染miR-17 mimics后,VSMC增殖能力增强,差异具有统计学意义(P<0.05),细胞周期G0/G1期明显减少,S与G2/M明显增加,差异具有统计学意义(P<0.05).与miR-17启动子区结合位点突变型(Mutant)组相比,转染miR-17启动子区野生型(Wild)组荧光素酶Luc活性增高,差异具有统计学意义(P<0.05).与对照组相比,LPS刺激细胞后,NF-κB p65、miR-17表达水平明显升高,差异具有统计学意义(P<0.05).结论 NF-κB通过直接结合miR-17启动子区,促进miR-17的表达从而促进VSMC的增殖.%Objective To investigate the role of miR-17 in the proliferation of vascular smooth muscle cells (VSMCs) and the mechanism of miR-17 regulated by nuclear factor (NF)-κB. Methods After miR-17 mimics were transfected into human vascular smooth muscle cells, and the cell viability was detected by CCK-8 and cell cycle was detected by flow cytometry. Bioinformatics predicted that the binding site between NF-κB p65 and miR-17 promoter region, and pcDNA3.1-p65 with reporter gene vector pGL3-miR-17 promoter region were co-transfected to 293T cells, and the effect of NF-κB p65 on miR-17 promoter region was analyzed by detecting the Luc activity of luciferase reporter gene. The expression of NF-κB p65 and miR-17 was detected after stimulated by LPS. Results Compared with miR-NC group, the proliferation of VSMC was enhanced after transfection with miR-17 mimics, with statistically difference (P<0.05) and cell cycle G0 / G1 phases were significantly decreased, while S and G2 /M phases were sig-nificantly increased, the difference were statistically significant (P<0.05). Compared with the pcDNA3.1-p65 Mutant group, the luciferase Luc activity of pcDNA3.1-p65Wild transfected group increased significantly (P<0.05). Compared with the control group, the expression of NF-κB p65 and miR-17 was significantly in-creased after stimulated by LPS (P<0.05). Conclusion NF-κB promotes the proliferation of VSMC by di-rectly binding to the miR-17 promoter region and promoting the expression of miR-17.